| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Simko, Paige E (16)
|
VAWST |
2:28.46 |
2:32.51 |
4.05 |
| 2 |
Warnagiris, Kimberly E (17)
|
VAWST |
2:28.34 |
2:38.41 |
10.07 |
| 3 |
Graves, Ciara H (15)
|
VATSU |
2:35.05 |
2:40.05 |
5.00 |
| 4 |
Masters, Lauren K (15)
|
VAWST |
2:30.24 |
2:41.88 |
11.64 |
| 5 |
Sprincis, Kayla M (16)
|
VAWST |
2:54.81 |
2:59.20 |
4.39 |
| 6 |
Tiberio, Kyleigh N (14)
|
VATSU |
2:58.62 |
3:00.57 |
1.95 |
| 7 |
Snapp, Leah K (16)
|
VAWST |
3:14.07 |
3:01.97 |
-12.10 |
| 8 |
Crampton, Isabella E (17)
|
VATSU |
3:01.16 |
3:02.75 |
1.59 |
| 9 |
Labrusciano, Anna F (14)
|
VATSU |
2:58.89 |
3:05.60 |
6.71 |
| 10 |
Shirk, Brianna M (13)
|
VAWST |
3:22.17 |
3:16.90 |
-5.27 |
| 11 |
Hartley, Niya D (13)
|
VAWST |
3:18.45 |
3:17.58 |
-0.87 |
| 12 |
Wilfong, Katherine S (13)
|
VAWST |
3:22.14 |
3:20.94 |
-1.20 |
| 13 |
Thomas, Claire O (14)
|
VATSU |
3:21.22 |
3:28.57 |
7.35 |
|
Burnes, Marah H (15)
|
VATORP |
3:11.91 |
DQ |
- |