| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gonzales, Lydia R (12)
|
MAWBY |
53.67 |
53.68 |
0.01 |
| 2 |
Folcomer, Tiffany P (12)
|
MAYY |
57.42 |
57.25 |
-0.17 |
| 3 |
Abel, Madelyn M (12)
|
MAUPY |
58.02 |
58.12 |
0.10 |
| 4 |
Kuteras, Victoria (12)
|
MAPFY |
57.72 |
58.46 |
0.74 |
| 5 |
Hinson, Kaliyah J (11)
|
MAYY |
55.96 |
58.57 |
2.61 |
| 6 |
Watschke, Madison L (12)
|
MASCAY |
58.77 |
58.74 |
-0.03 |
| 7 |
Hidalgo, Carla J (12)
|
MALEBY |
58.33 |
59.52 |
1.19 |
| 8 |
Williams, Lydia E (12)
|
MAYY |
1:00.49 |
1:00.28 |
-0.21 |
| 9 |
Keefer, Morgan E (12)
|
MAWSY |
1:00.64 |
1:01.21 |
0.57 |
| 10 |
Del Rosario, Xiomara (12)
|
MACC |
1:01.26 |
1:01.41 |
0.15 |
| 11 |
Miller, Brianna E (11)
|
MAYY |
59.50 |
1:01.68 |
2.18 |
| 12 |
Markle, Lauren (12)
|
MACC |
1:01.68 |
1:01.71 |
0.03 |
| 13 |
Updegrave, Delaney M (12)
|
MANDCY |
1:01.68 |
1:01.73 |
0.05 |
| 14 |
Evans, Lilly (12)
|
MACC |
1:01.59 |
1:02.55 |
0.96 |
| 15 |
Durr, Madeline W (12)
|
MANEYS |
1:05.98 |
1:03.53 |
-2.45 |
| 16 |
Giesselbach, Sarah (12)
|
MAYY |
1:03.90 |
1:04.33 |
0.43 |
| 17 |
Ludwick, Chloe M (11)
|
MAWSY |
1:05.27 |
1:05.04 |
-0.23 |
| 18 |
Scarcella, Gianna (12)
|
MAFAY |
1:06.18 |
1:05.75 |
-0.43 |
| 19 |
Delaney, Charlotte (11)
|
MASCAY |
1:06.94 |
1:06.29 |
-0.65 |
| 20 |
Martinko, Elizabeth (12)
|
MASKY |
1:06.68 |
1:06.46 |
-0.22 |
| 21 |
Christensen, Elle (11)
|
LHY |
1:07.40 |
1:06.56 |
-0.84 |
| 22 |
Wolfe, Ashlynn (12)
|
MAWBY |
1:06.40 |
1:07.31 |
0.91 |
| 23 |
Robbins, Makenna (11)
|
JSY |
1:06.90 |
1:07.55 |
0.65 |
| 24 |
Levy, Grace S (12)
|
MASCAY |
1:05.45 |
1:07.90 |
2.45 |
| 25 |
Klena, Maria (12)
|
ENBEFY |
1:07.23 |
1:08.18 |
0.95 |
| 26 |
Svasrstad, Tuva S (12)
|
ZZCYAA |
1:05.03 |
1:09.71 |
4.68 |