| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Powers, Addison R (12)
|
MDMAS |
5:13.19 |
5:07.55 |
-5.64 |
| 2 |
Carliss, Sophia E (12)
|
MDFAST |
5:18.67 |
5:21.12 |
2.45 |
| 3 |
Schanamann, Danielle K (12)
|
MDMAS |
|
5:30.74 |
- |
| 4 |
Stephan, Ainsley M (11)
|
MDCAA |
6:36.96 |
5:37.39 |
-59.57 |
| 5 |
Pingaro, Regen O (11)
|
MDCAA |
5:51.36 |
5:47.55 |
-3.81 |
| 6 |
Welker, Harper K (12)
|
MDFAST |
5:55.91 |
5:52.80 |
-3.11 |
| 7 |
Gillum, Tai D (12)
|
MDCAA |
6:04.79 |
6:02.22 |
-2.57 |
| 8 |
Harshman, Madeline M (12)
|
MDFAST |
6:24.28 |
6:04.29 |
-19.99 |
| 9 |
Liu, Emma W (11)
|
MDCAA |
6:19.32 |
6:07.90 |
-11.42 |
| 10 |
Young, Alyssa L (12)
|
MDCAA |
6:11.58 |
6:19.56 |
7.98 |
| 11 |
Wainberg, Brianna P (12)
|
MDFAST |
6:43.05 |
6:49.45 |
6.40 |