| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chen, Gwyneth E (11)
|
GUUN |
5:15.09 |
5:12.89 |
-2.20 |
| 2 |
Rayburn, Myah A (12)
|
GUUN |
5:19.39 |
5:17.01 |
-2.38 |
| 3 |
Upton, Madisyn R (11)
|
NCYBAC |
5:28.26 |
5:24.52 |
-3.74 |
| 4 |
Dyo, Cara K (11)
|
GUESCA |
5:41.99 |
5:27.30 |
-14.69 |
| 5 |
Jandera-Chambless, Sofia I (11)
|
NCHHST |
5:38.35 |
5:29.03 |
-9.32 |
| 6 |
Meislahn, Kinley A (12)
|
FLLRSA |
5:49.50 |
5:29.35 |
-20.15 |
| 7 |
Silvagnoli, Iara V (12)
|
NCYBAC |
5:28.23 |
5:40.16 |
11.93 |
| 8 |
Hall, Blake L (12)
|
ARSWST |
6:10.51 |
6:11.62 |
1.11 |
| 9 |
Chrisman, Alexandra (12)
|
ARTEAM |
6:22.97 |
6:16.10 |
-6.87 |
|
Lombardo, Michaele (11)
|
FLBA |
6:45.74 |
NS |
- |
|
Rodriguez, Islabela C (12)
|
GUUN |
6:21.23 |
NS |
- |