| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chen, Gwyneth E (11)
|
GUUN |
2:27.89 |
2:27.66 |
-0.23 |
| 2 |
Chavis, Adriana (12)
|
ZZARA |
2:34.35 |
2:30.59 |
-3.76 |
| 3 |
Jandera-Chambless, Sofia I (11)
|
NCHHST |
2:37.40 |
2:31.20 |
-6.20 |
| 4 |
Rayburn, Myah A (12)
|
GUUN |
2:35.89 |
2:31.58 |
-4.31 |
| 5 |
Dyo, Cara K (11)
|
GUESCA |
2:42.28 |
2:37.59 |
-4.69 |
| 6 |
Strong, Jordyn G (12)
|
GUTASC |
2:49.23 |
2:38.46 |
-10.77 |
| 7 |
Upton, Madisyn R (11)
|
NCYBAC |
2:36.61 |
2:40.19 |
3.58 |
| 8 |
Silvagnoli, Iara V (12)
|
NCYBAC |
2:36.63 |
2:43.26 |
6.63 |
| 9 |
Meislahn, Kinley A (12)
|
FLLRSA |
2:42.18 |
2:43.68 |
1.50 |
| 10 |
Hamilton, Dalyana C (12)
|
NENE |
2:24.99 |
2:43.95 |
18.96 |
| 11 |
Principe, Ella J (11)
|
FLBW |
2:40.78 |
2:45.15 |
4.37 |
| 12 |
Lester, Peyton J (11)
|
FLBW |
2:49.24 |
2:46.44 |
-2.80 |
| 13 |
White, Victoria (12)
|
FLFL |
2:50.05 |
2:47.63 |
-2.42 |
| 14 |
Torres, Sophia (11)
|
FLBW |
2:58.96 |
2:48.87 |
-10.09 |
| 15 |
Hall, Blake L (12)
|
ARSWST |
3:09.08 |
2:51.85 |
-17.23 |
| 16 |
Rodriguez, Islabela C (12)
|
GUUN |
2:53.27 |
2:52.16 |
-1.11 |
| 17 |
McGee, EmilyPaige E (12)
|
GULSST |
2:41.53 |
2:52.71 |
11.18 |
| 18 |
Hall, Allie (11)
|
ARTEAM |
2:49.62 |
2:53.49 |
3.87 |
| 19 |
Jerichow, Jayana (11)
|
FLFL |
2:35.21 |
2:54.21 |
19.00 |
| 20 |
Langston, Sydney G (12)
|
OZSMSC |
3:01.54 |
2:55.48 |
-6.06 |
| 21 |
Williams, Amelia J (11)
|
ARSWST |
3:08.37 |
2:58.22 |
-10.15 |
| 22 |
Chrisman, Alexandra (12)
|
ARTEAM |
2:38.03 |
2:59.17 |
21.14 |
| 23 |
Carpenter, Cora (12)
|
ARTEAM |
3:04.09 |
3:03.49 |
-0.60 |
| 24 |
Cossey, Hannah (12)
|
ARTEAM |
3:04.04 |
3:14.03 |
9.99 |
| 25 |
Weibrecht, Addison (12)
|
OZSMSC |
3:41.70 |
3:14.48 |
-27.22 |
| 26 |
Perez, Solymar (11)
|
FLBA |
3:15.71 |
3:14.57 |
-1.14 |