| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wales, Nathaniel J (15)
|
NISYCH |
24.19 |
24.33 |
0.14 |
| 2 |
Hicks, Andrew T (16)
|
NISYCH |
24.60 |
24.43 |
-0.17 |
| 3 |
Wratney, Elise C (13)
|
NISYCH |
26.99 |
27.38 |
0.39 |
| 4 |
Gabrielli, Anthony J (15)
|
NISYCH |
|
27.78 |
- |
| 5 |
Butler, Ruby C (13)
|
NISYCH |
|
29.09 |
- |
| 6 |
Butler, Rainbow C (13)
|
NISYCH |
|
29.24 |
- |
| 7 |
Fuller, Anna G (12)
|
NISYCH |
30.54 |
29.99 |
-0.55 |
| 8 |
Francescotti, Maria G (14)
|
NISYCH |
31.16 |
30.45 |
-0.71 |
| 9 |
Liu, Kelly Y (13)
|
NISYCH |
|
30.62 |
- |
| 10 |
Ottaviano, Catherine E (18)
|
NISYCH |
28.55 |
31.41 |
2.86 |
| 11 |
Wheeler, Samantha A (12)
|
NISYCH |
|
32.86 |
- |
| 12 |
Luttrell, Avery M (14)
|
NISYCH |
37.29 |
35.47 |
-1.82 |