| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McDevitt, Elizabeth R (17)
|
NCYBAC |
5:18.90 |
5:29.40 |
10.50 |
| 2 |
Halvorson, Samantha E (15)
|
VAECAT |
5:57.90 |
5:33.64 |
-24.26 |
| 3 |
Sciaudone, Rose M (16)
|
NCMOR |
5:39.83 |
5:33.79 |
-6.04 |
| 4 |
Jones, Chelsey M (16)
|
VAVSTP |
5:19.34 |
5:44.86 |
25.52 |
| 5 |
Xu, Amanda T (16)
|
MDCAA |
5:06.80 |
5:46.62 |
39.82 |
| 6 |
Graves, Ciara H (16)
|
VATSU |
5:39.69 |
5:48.95 |
9.26 |
| 7 |
Russin, Ellie M (17)
|
NCMOR |
5:54.87 |
5:54.62 |
-0.25 |
| 8 |
Long, Maya E (15)
|
VAPSDN |
5:02.78 |
5:56.92 |
54.14 |
| 9 |
Parker, Morgan R (15)
|
VARAYS |
6:12.04 |
6:02.04 |
-10.00 |
| 10 |
Carter, Braylee M (16)
|
NCYBAC |
5:41.31 |
6:03.08 |
21.77 |
| 11 |
Green, Emma E (16)
|
VARAYS |
5:43.68 |
6:04.64 |
20.96 |
| 12 |
Campbell, Madelyn L (17)
|
VAPSDN |
6:00.67 |
6:08.94 |
8.27 |
| 13 |
Giovinazzo, Alyssa N (15)
|
VAPWSC |
6:36.11 |
6:12.95 |
-23.16 |
|
Dyl, Madison N (15)
|
VATSU |
|
DQ |
- |