| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trutschl, Maya J (13)
|
LAYNWL |
33.26 |
30.41 |
-2.85 |
| 2 |
Montier, Jennifer (14)
|
NTMTRO |
35.20 |
30.51 |
-4.69 |
| 3 |
Drozdz, Julia B (14)
|
LAYNWL |
31.14 |
31.25 |
0.11 |
| 4 |
Gill, Brooke K (13)
|
LACOLA |
32.89 |
31.26 |
-1.63 |
| 5 |
Roper, Claire M (14)
|
LAYNWL |
31.83 |
31.44 |
-0.39 |
| 6 |
Lemmert, Ellie C (13)
|
NTTRAC |
32.47 |
31.65 |
-0.82 |
| 7 |
Taylor, Alexandra K (13)
|
LAYNWL |
33.10 |
31.81 |
-1.29 |
| 7 |
Glass, Ella A (14)
|
LAYNWL |
34.24 |
31.81 |
-2.43 |
| 9 |
Vo, Jolie M (13)
|
LAYNWL |
33.19 |
31.97 |
-1.22 |
| 10 |
Hamilton, Isabella E (14)
|
LACLAC |
32.03 |
32.49 |
0.46 |
| 11 |
Olson, Grace E (13)
|
NTMTRO |
33.80 |
32.81 |
-0.99 |
| 12 |
Heflin, Sara G (13)
|
LAYNWL |
35.46 |
33.97 |
-1.49 |
| 13 |
Greene, Avagail K (14)
|
NTTRAC |
33.99 |
34.64 |
0.65 |
| 14 |
Finehirsh, Madeleine B (13)
|
LACOLA |
|
34.76 |
- |
| 15 |
Pizarro, Elena M (14)
|
LAYNWL |
36.26 |
35.54 |
-0.72 |
| 16 |
Tichenor, Jennie T (13)
|
LACLAC |
39.60 |
35.61 |
-3.99 |
| 17 |
Howard, Hannah M (13)
|
LACLAC |
|
35.70 |
- |
| 18 |
Green, Elizabeth T (13)
|
LAYNWL |
|
36.50 |
- |
| 19 |
Falgout, Allie R (13)
|
LACOLA |
37.73 |
36.99 |
-0.74 |
| 20 |
LaCombe-Bennett, Bailey A (14)
|
LACOAS |
40.04 |
37.97 |
-2.07 |
| 21 |
Rodgers, Jillian K (13)
|
LACLAC |
|
37.99 |
- |
|
Lipp, Michaela (14)
|
NTMTRO |
34.67 |
NS |
- |