| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lally, Keely M (12)
|
MRFREE |
29.16 |
28.61 |
-0.55 |
| 2 |
Roudnitsky, Julia (12)
|
MRMPNY |
30.53 |
29.86 |
-0.67 |
| 3 |
Levitski, Katherina A (12)
|
MRFREE |
31.44 |
30.28 |
-1.16 |
| 4 |
Ilyusha, Sophie (12)
|
MRMPNY |
29.79 |
30.73 |
0.94 |
| 5 |
Reid, Morgan N (12)
|
MRFREE |
32.87 |
32.32 |
-0.55 |
| 6 |
Perpignan, Marleigh A (12)
|
MRFREE |
34.72 |
32.80 |
-1.92 |
| 7 |
Velasquez, Luna M (12)
|
MRCENT |
45.00 |
36.20 |
-8.80 |
| 8 |
Rodriguez, Aliya (12)
|
MRCENT |
42.19 |
38.50 |
-3.69 |
| 9 |
Roberts, Maya (12)
|
MRCENT |
45.00 |
39.31 |
-5.69 |
| 10 |
Olszowka, Lena (12)
|
MRCENT |
43.12 |
39.47 |
-3.65 |
| 11 |
Bryson, Naia (12)
|
MRCENT |
45.00 |
39.72 |
-5.28 |