| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bendersky, Hailey R (10)
|
MRWISC |
33.53 |
32.57 |
-0.96 |
| 2 |
Behrend, Molly C (10)
|
MRWEST |
34.39 |
32.63 |
-1.76 |
| 3 |
Blaho, Fiona A (9)
|
MREAST |
34.96 |
32.97 |
-1.99 |
| 4 |
DeCasseres, Brianna O (10)
|
MRWEST |
32.73 |
33.03 |
0.30 |
| 5 |
Bentley, Elena M (10)
|
MREAST |
35.95 |
34.80 |
-1.15 |
| 6 |
Meliere, Lily M (10)
|
MRWISC |
37.58 |
34.82 |
-2.76 |
| 7 |
Galimi, Maeve O (10)
|
MRWEST |
36.31 |
35.37 |
-0.94 |
| 8 |
Shlivko, Yuri (9)
|
MRMPNY |
36.57 |
35.87 |
-0.70 |
| 9 |
Sherman, Alexa L (10)
|
MRLIE |
40.24 |
35.92 |
-4.32 |
| 10 |
Javer, Samara L (10)
|
MREAST |
37.02 |
36.38 |
-0.64 |
| 11 |
Jia, Nicole X (10)
|
MRMPNY |
37.00 |
39.13 |
2.13 |
| 12 |
McMahon, Reilly E (10)
|
MRWISC |
38.65 |
39.68 |
1.03 |
| 13 |
Yu, Charis (10)
|
MRMPNY |
43.19 |
39.79 |
-3.40 |
| 14 |
Mancuso, Piper T (8)
|
MRLIE |
49.07 |
43.26 |
-5.81 |
| 15 |
Shanley, Clara P (10)
|
MRWEST |
46.04 |
44.13 |
-1.91 |
| 16 |
Molina, Isabella E (10)
|
MRLIFE |
40.85 |
44.44 |
3.59 |
| 17 |
Kofsky, Alexandra I (9)
|
MRWISC |
55.99 |
47.99 |
-8.00 |
| 18 |
Krapaney, Julia (9)
|
MRMPNY |
56.27 |
49.14 |
-7.13 |
| 19 |
Yang, Michelle (10)
|
MRLIE |
|
51.33 |
- |
| 20 |
Smith, Elsie (10)
|
MRLIE |
|
55.41 |
- |
| 21 |
Djokovic-Farrugia, Lina M (10)
|
MREAST |
55.00 |
1:01.24 |
6.24 |
|
Cokley, Isabelle E (8)
|
MRLIE |
|
NS |
- |