| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lam, Kylie L (12)
|
MRQNS |
29.75 |
29.84 |
0.09 |
| 2 |
Harkins, Morgan P (12)
|
MRLIE |
33.50 |
31.63 |
-1.87 |
| 3 |
Akmatbaeva, Deniz (12)
|
MRQNS |
32.55 |
31.98 |
-0.57 |
| 4 |
Davis, Naomi (12)
|
MRCSDC |
33.19 |
32.16 |
-1.03 |
| 5 |
Kang, Sunny (11)
|
MRWEST |
32.81 |
32.23 |
-0.58 |
| 6 |
LaLota, Maggie R (11)
|
MRWISC |
36.01 |
33.71 |
-2.30 |
| 7 |
Giagios, Andraya B (12)
|
MRSSC |
35.98 |
34.02 |
-1.96 |
| 8 |
Borowski, Elaina (11)
|
MRCSDC |
40.87 |
34.15 |
-6.72 |
| 9 |
Brodsky, Aliyah R (11)
|
MRLIE |
|
34.25 |
- |
| 10 |
Ali, Katina L (12)
|
MRWISC |
34.90 |
34.66 |
-0.24 |
| 11 |
Grotto, Gianna R (12)
|
MRSSC |
35.36 |
34.85 |
-0.51 |
| 12 |
Vardoulias, Sofia (12)
|
MRLIE |
42.54 |
35.32 |
-7.22 |
| 13 |
Clarke, Hailey E (12)
|
MRLIE |
|
35.61 |
- |
| 14 |
Cagno, Lucia P (12)
|
MRQNS |
35.88 |
35.87 |
-0.01 |
| 14 |
O'Sullivan, Brinan K (11)
|
MRQNS |
36.30 |
35.87 |
-0.43 |
| 16 |
McKiernan, Taryn (11)
|
MRCSDC |
|
35.91 |
- |
| 17 |
Canon, Sophia H (12)
|
MRLIE |
|
36.40 |
- |
| 18 |
Singh, Chloe M (11)
|
MRSSC |
37.24 |
36.85 |
-0.39 |
| 19 |
Hunter, Madeleine J (11)
|
MRLIE |
53.53 |
38.02 |
-15.51 |
| 20 |
Lemaitre, Nina J (11)
|
MRLIE |
|
38.38 |
- |
| 21 |
Colletti, Sofia (12)
|
MRLIE |
44.12 |
39.41 |
-4.71 |
| 22 |
Colletti, Claire J (12)
|
MRLIE |
41.82 |
39.66 |
-2.16 |
| 23 |
Garra, Isabella G (12)
|
MRLIE |
42.27 |
39.81 |
-2.46 |
| 24 |
Mongello, Catherine A (12)
|
MRLIE |
|
40.56 |
- |
| 25 |
Walsh, Julianne L (11)
|
MRLIE |
|
42.69 |
- |
| 26 |
Azzollini, Elisabeth H (11)
|
MRCSDC |
|
43.45 |
- |
| 27 |
McShane, Mollie E (11)
|
MRLIE |
|
45.65 |
- |
| 28 |
Raut, Jiuika M (12)
|
MRLIE |
|
48.70 |
- |