| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hall, Justin R (12)
|
MDRAC |
26.88 |
27.24 |
0.36 |
| 2 |
Pearson, Emmanuel Q (12)
|
MDSMDA |
29.69 |
27.70 |
-1.99 |
| 3 |
Hughes, Jonathan D (12)
|
MDNBAC |
27.98 |
28.36 |
0.38 |
| 4 |
Seavers-Reale, Bennett E (12)
|
MDNBAC |
29.13 |
29.02 |
-0.11 |
| 5 |
Dorsey, David R (12)
|
MDRAC |
29.07 |
29.22 |
0.15 |
| 6 |
Asekun, Ayobami J (12)
|
MDRAC |
31.63 |
30.30 |
-1.33 |
| 7 |
Chiang, Isaac I (11)
|
MDNBAC |
30.70 |
30.59 |
-0.11 |
| 8 |
McQuaid, Brady P (11)
|
MDNBAC |
30.90 |
30.87 |
-0.03 |
| 9 |
Govern, Aiden C (12)
|
MDBAY |
31.76 |
31.98 |
0.22 |
| 10 |
Nanaji, Omid (11)
|
MDRAC |
32.39 |
32.48 |
0.09 |
| 11 |
Ritchey, Alexander W (12)
|
MDCAA |
|
32.52 |
- |
| 12 |
Suprun, Maximilian E (11)
|
MDCAA |
33.97 |
33.76 |
-0.21 |
| 13 |
Shedwick, Owen C (12)
|
MDNBAC |
36.66 |
34.09 |
-2.57 |
| 14 |
Spotte, Adler B (11)
|
MDNBAC |
33.94 |
34.11 |
0.17 |
| 15 |
Bowman, Austin P (11)
|
MDRAC |
34.16 |
35.01 |
0.85 |
| 16 |
Stanley, Oliver S (11)
|
MDNBAC |
35.40 |
35.16 |
-0.24 |
| 17 |
Barnett, Jason R (12)
|
MDNBAC |
34.54 |
35.30 |
0.76 |
| 18 |
Greenberger, Seth D (12)
|
MDCAA |
34.81 |
35.34 |
0.53 |
| 19 |
Kibler, John A (11)
|
MDBAY |
39.11 |
36.92 |
-2.19 |
| 20 |
Shepelevych, Nazar (11)
|
MDRAC |
37.75 |
37.29 |
-0.46 |
| 21 |
Pearson, Levi E (11)
|
MDSMDA |
42.64 |
39.30 |
-3.34 |
| 22 |
Romero, Gabriel C (12)
|
MDNBAC |
37.88 |
39.65 |
1.77 |
| 23 |
Liu, Ethan (11)
|
MDRAC |
|
39.88 |
- |
| 24 |
Barbaris, Robert A (12)
|
MDACA |
38.44 |
40.52 |
2.08 |
| 25 |
Hupert, Spencer J (11)
|
MDSMDA |
49.77 |
44.86 |
-4.91 |
|
Eppley-Pelusi, Sage Z (11)
|
MDRAC |
|
NS |
- |
|
Trainor, William C (11)
|
MDNBAC |
34.82 |
NS |
- |
|
Gilkis, David J (11)
|
MDNBAC |
39.36 |
NS |
- |
|
Le, Maxwell T (12)
|
MDACA |
38.57 |
NS |
- |