| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Davis, Elise C (10)
|
NCYOTA |
40.16 |
39.21 |
-0.95 |
| 2 |
Kistler, Shelby N (10)
|
NCYBAC |
40.30 |
39.54 |
-0.76 |
| 3 |
McCowen, Sarah E (10)
|
NCYOTA |
42.73 |
40.24 |
-2.49 |
| 4 |
Volpe, Annalisa G (10)
|
NCYOTA |
41.75 |
42.09 |
0.34 |
| 5 |
Gutierrez, Rosario L (10)
|
NCYOTA |
47.74 |
43.35 |
-4.39 |
| 6 |
Smitherman, Katherine M (10)
|
NCTYDE |
46.47 |
43.92 |
-2.55 |
| 7 |
Barritt, Helen E (10)
|
NCYOTA |
42.00 |
44.19 |
2.19 |
| 8 |
Mckinnon, Rachel G (9)
|
NCTYDE |
46.31 |
44.51 |
-1.80 |
| 9 |
Herlocker, Patterson L (10)
|
NCRACY |
45.34 |
44.53 |
-0.81 |
| 10 |
Keough, Alannah K (9)
|
NCYOTA |
43.88 |
44.93 |
1.05 |
| 11 |
Powell, Catherine G (10)
|
NCYOTA |
48.42 |
45.30 |
-3.12 |
| 12 |
Stasko, Natalie J (10)
|
NCYOTA |
47.55 |
45.55 |
-2.00 |
| 13 |
Drozd, Charlotte A (9)
|
NCLOY |
50.78 |
47.14 |
-3.64 |
| 14 |
Brewer, Reagan N (9)
|
NCRMY |
51.61 |
49.70 |
-1.91 |