| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cummins, Bodhi K (10)
|
NCUN |
29.10 |
28.84 |
-0.26 |
| 2 |
Dickenson, Anderson D (9)
|
NCSTAR |
32.56 |
31.70 |
-0.86 |
| 3 |
Simon, Eli T (10)
|
NCTYDE |
34.88 |
34.00 |
-0.88 |
| 4 |
Simon, Caleb J (9)
|
NCTYDE |
35.45 |
34.92 |
-0.53 |
| 5 |
Shytle, William J (9)
|
NCTYDE |
37.53 |
35.82 |
-1.71 |
| 6 |
Crowley, George L (10)
|
NCTYDE |
35.20 |
35.83 |
0.63 |
| 7 |
Hennis, Liam R (8)
|
NCTYDE |
40.45 |
38.47 |
-1.98 |
| 8 |
Holmes, Robert C (9)
|
NCTYDE |
38.67 |
39.35 |
0.68 |
| 9 |
Burgess-Hicks, Kal Hyun N (10)
|
NCGCY |
44.14 |
39.71 |
-4.43 |
| 10 |
Yates, Braylen S (9)
|
NCRACY |
37.87 |
42.25 |
4.38 |
| 11 |
Snapp, Lucas A (7)
|
NCTYDE |
|
42.63 |
- |
| 12 |
Brinkley, Nolan C (8)
|
NCRACY |
46.99 |
42.92 |
-4.07 |
| 13 |
Dinkins, Elijah D (8)
|
NCTYDE |
|
44.59 |
- |
| 14 |
Cook, William H (9)
|
NCSSS |
|
44.84 |
- |
| 15 |
Muirhead, Ayden G (8)
|
NCSGSA |
43.68 |
47.43 |
3.75 |
| 16 |
McCall, William H (10)
|
NCSSS |
|
47.66 |
- |
| 17 |
Burns, Alexander P (8)
|
NCTYDE |
49.98 |
48.62 |
-1.36 |
| 18 |
Wheeler, Colt J (8)
|
NCTYDE |
47.50 |
50.11 |
2.61 |