| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hammons, Kelsey M (17)
|
OHRCDB |
25.72 |
25.98 |
0.26 |
| 2 |
Williams, Alivia L (18)
|
OHRCDB |
26.08 |
26.56 |
0.48 |
| 3 |
Ackerman, Claire L (15)
|
KYBYB |
25.37 |
26.64 |
1.27 |
| 4 |
Rice, Lily A (17)
|
OHRCDB |
26.66 |
26.78 |
0.12 |
| 5 |
Hetman, Lauren E (18)
|
OHCFY |
26.15 |
27.09 |
0.94 |
| 6 |
Voisinet, Carsen L (15)
|
OHMCY |
27.80 |
28.35 |
0.55 |
| 7 |
Sunvold, Rebekah (16)
|
OHPCYP |
29.43 |
28.74 |
-0.69 |
| 8 |
Sickmeier, Maria T (15)
|
OHRCDB |
28.14 |
28.96 |
0.82 |
| 9 |
House, Paige E (15)
|
OHPCYP |
29.07 |
29.02 |
-0.05 |
| 10 |
Faulconer, Lizzie M (17)
|
KYBYB |
28.11 |
29.34 |
1.23 |
| 11 |
Przanowski, Caylissa J (15)
|
OHRCDB |
27.88 |
29.87 |
1.99 |
| 12 |
Cagle, Abigail R (16)
|
OHPCY |
28.35 |
30.13 |
1.78 |
| 13 |
Clarke, Georgia E (16)
|
KYBYB |
29.20 |
30.45 |
1.25 |
| 14 |
Stevens, Kilee A (16)
|
KYBYB |
28.94 |
30.61 |
1.67 |
| 15 |
Jones, Anna C (16)
|
KYBYB |
29.82 |
30.88 |
1.06 |
| 16 |
Schneider, Maggie A (17)
|
KYBYB |
28.18 |
31.87 |
3.69 |
| 17 |
Hunt, Maris (16)
|
INYOGI |
30.77 |
32.14 |
1.37 |
| 18 |
Vander Veen, Kelly J (15)
|
OHPCY |
34.65 |
34.01 |
-0.64 |
| 19 |
Berry, Elise (18)
|
INYOGI |
41.31 |
36.36 |
-4.95 |
| 20 |
Kavanaugh, Carly E (15)
|
OHLAKY |
36.70 |
36.82 |
0.12 |
|
Ryan, Sophie A (17)
|
OHRCDB |
27.21 |
NS |
- |
|
Williams, Makenna G (15)
|
OHABLY |
27.20 |
NS |
- |
|
Carman, Isabella (15)
|
INYOGI |
37.16 |
NS |
- |
|
Carman, Sophia (18)
|
INYOGI |
50.98 |
NS |
- |
|
Peck, Lauren G (17)
|
KYBYB |
30.19 |
NS |
- |