| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brown, Jack H (15)
|
OHRCDB |
54.16 |
54.34 |
0.18 |
| 2 |
Furuta, Roman R (16)
|
OHRCDB |
55.76 |
54.79 |
-0.97 |
| 3 |
Koenigsknecht, Benett D (17)
|
OHRCDB |
56.18 |
56.29 |
0.11 |
| 4 |
Walter, Aiden A (15)
|
OHLAKY |
59.32 |
58.54 |
-0.78 |
| 5 |
Earls, Avery C (15)
|
OHGMVY |
59.10 |
59.80 |
0.70 |
| 6 |
Dattilio, Mark L (16)
|
KYBYB |
1:01.72 |
1:00.50 |
-1.22 |
| 7 |
Pache, Daniel (18)
|
INYOGI |
1:01.35 |
1:01.20 |
-0.15 |
| 8 |
Morgan IV, Jesse D (18)
|
OHHHY |
1:00.45 |
1:01.60 |
1.15 |
| 9 |
Richter, Camden J (17)
|
OHMCY |
1:02.98 |
1:04.66 |
1.68 |
| 10 |
Christenson, Matthew D (16)
|
OHMCY |
1:02.60 |
1:05.50 |
2.90 |
| 11 |
Higgs, Harrison (17)
|
KYBYB |
1:04.17 |
1:07.06 |
2.89 |
| 12 |
Barker, Nathaniel L (15)
|
KYBYB |
1:16.03 |
1:14.36 |
-1.67 |
|
Galbraith, Ruarigh P (15)
|
OHCFY |
1:00.75 |
NS |
- |