| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fox, Chloe G (10)
|
OHPCY |
49.67 |
40.90 |
-8.77 |
| 2 |
Howell, Skylar R (10)
|
OHKEY |
50.01 |
41.37 |
-8.64 |
| 3 |
Adams, Willow E (10)
|
OHPCY |
|
42.05 |
- |
| 4 |
Johnson, Maggie G (9)
|
OHRCDB |
|
47.12 |
- |
| 5 |
Diederichs, Eva J (10)
|
OHABLY |
|
47.25 |
- |
| 6 |
Ripley, Taryn E (10)
|
OHABLY |
49.72 |
50.83 |
1.11 |
| 7 |
Hill, Elizabeth J (10)
|
OHSPY |
|
52.22 |
- |
| 8 |
Gholston, Talia K (10)
|
OHPCY |
|
52.52 |
- |
| 9 |
Sutphin, Isla S (9)
|
OHABLY |
1:03.65 |
54.11 |
-9.54 |
| 10 |
Lucas, Annie S (9)
|
OHRCDB |
1:21.60 |
54.97 |
-26.63 |
| 11 |
Raimondo, Gemma L (9)
|
OHABLY |
56.36 |
57.18 |
0.82 |
| 12 |
Pan, Lyla L (9)
|
OHBASH |
1:14.35 |
57.28 |
-17.07 |
| 13 |
Stuckey, Allie M (9)
|
OHSPY |
|
57.42 |
- |
| 14 |
Clark, Aubrey P (10)
|
OHSPY |
1:09.60 |
57.65 |
-11.95 |
| 15 |
Coughenour, Harper G (9)
|
OHRCDB |
1:07.08 |
58.87 |
-8.21 |
| 16 |
Khadka, Sana S (10)
|
OHBASH |
1:22.87 |
1:07.42 |
-15.45 |
|
Gozal, Olivia A (9)
|
OHPCY |
57.26 |
DQ |
- |
|
Mount, Jocelyn R (10)
|
OHBASH |
|
DQ |
- |
|
Kawala, Milena A (10)
|
OHBASH |
|
DQ |
- |
|
Garnica, Immanuelle L (10)
|
OHBASH |
|
DQ |
- |