| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Susec, Abigail D (18)
|
OHRAYS |
31.21 |
30.43 |
-0.78 |
| 2 |
Connolly, Katherine P (20)
|
OHCATS |
30.40 |
30.68 |
0.28 |
| 3 |
Pivor, Isabella R (17)
|
OHBASH |
30.33 |
31.03 |
0.70 |
| 4 |
Robillard, Adalene L (13)
|
OHRAYS |
33.34 |
31.99 |
-1.35 |
| 5 |
Jantausch, Elizabeth A (19)
|
OHCATS |
30.61 |
32.00 |
1.39 |
| 6 |
Hunt, Victoria M (21)
|
OHCATS |
30.13 |
32.42 |
2.29 |
| 7 |
Menner, Zion A (14)
|
OHRAYS |
36.34 |
34.94 |
-1.40 |
| 8 |
Hubbell, Keally P (13)
|
OHBASH |
41.17 |
37.74 |
-3.43 |
| 9 |
Koetter, Elizabeth S (16)
|
OHBASH |
37.18 |
37.92 |
0.74 |
| 10 |
Rubi, Lola I (12)
|
OHPCY |
46.55 |
38.58 |
-7.97 |
| 11 |
Wallbrown, Katherine G (12)
|
OHBASH |
46.28 |
38.60 |
-7.68 |
| 12 |
Lewis, Helen K (18)
|
OHBASH |
38.02 |
39.67 |
1.65 |