| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Balcerak, Michael A (21)
|
OHCATS |
55.26 |
56.02 |
0.76 |
| 2 |
White, Kyle S (21)
|
OHCATS |
56.12 |
58.31 |
2.19 |
| 3 |
Paxton, Christopher L (18)
|
OHPCY |
56.33 |
59.53 |
3.20 |
| 4 |
Froass, William P (17)
|
OHRAYS |
58.75 |
59.82 |
1.07 |
| 5 |
Christensen, Maxwell A (16)
|
OHRAYS |
1:08.67 |
1:02.58 |
-6.09 |
| 6 |
Zhao, Jason J (14)
|
OHBASH |
1:12.97 |
1:04.96 |
-8.01 |
| 7 |
Valentine, Joshua M (18)
|
OHPCY |
1:02.91 |
1:06.04 |
3.13 |
| 8 |
Blazer, Evan J (16)
|
OHSPY |
1:06.89 |
1:06.49 |
-0.40 |
| 9 |
Myers, Braydon T (15)
|
OHPCY |
|
1:09.00 |
- |
| 10 |
Miller, Evan J (16)
|
OHPCY |
1:13.66 |
1:10.67 |
-2.99 |
| 11 |
Schramm, Robert J (20)
|
OHBASH |
1:07.23 |
1:11.15 |
3.92 |
| 12 |
Stakauskas, Andrew W (15)
|
OHPCY |
|
1:11.67 |
- |
| 13 |
Akemon, Bryce M (15)
|
OHPCY |
1:15.26 |
1:12.96 |
-2.30 |
| 14 |
Cross, Hendrix J (14)
|
OHPCY |
1:23.62 |
1:13.72 |
-9.90 |
| 15 |
Brady, Noah S (14)
|
OHBASH |
1:21.16 |
1:15.23 |
-5.93 |
| 16 |
Hagenauer, Carter F (14)
|
OHPCY |
1:17.71 |
1:15.50 |
-2.21 |
| 17 |
Spivery, Noah S (13)
|
OHBASH |
1:25.72 |
1:19.05 |
-6.67 |
| 18 |
Lewis, Robert K (13)
|
OHBASH |
1:20.50 |
1:19.41 |
-1.09 |
| 19 |
Scherz II, Thomas C (16)
|
OHPCY |
|
1:21.66 |
- |
| 20 |
Ranly, Austin J (12)
|
OHBASH |
1:32.03 |
1:24.44 |
-7.59 |
|
Sequeira, Aaron M (19)
|
OHCM |
53.85 |
NS |
- |