| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nguyen, Sloane M (8)
|
PVMACH |
34.57 |
33.21 |
-1.36 |
| 2 |
Iannitto, Emerson G (8)
|
PVNCAP |
36.69 |
37.68 |
0.99 |
| 3 |
Vanlandingham, Quinn O (8)
|
PVNCAP |
39.55 |
38.34 |
-1.21 |
| 4 |
Baker, Violet E (8)
|
PVMACH |
41.33 |
38.36 |
-2.97 |
| 5 |
Shoemaker, Emily M (8)
|
PVNCAP |
38.31 |
38.42 |
0.11 |
| 6 |
Alex, Kenzie M (8)
|
PVNCAP |
45.00 |
38.50 |
-6.50 |
| 7 |
Vayra, Eva T (8)
|
PVNCAP |
|
40.71 |
- |
| 8 |
Tofilon, Sophie J (8)
|
PVNCAP |
45.48 |
42.17 |
-3.31 |
| 9 |
Matteo, Ariana (8)
|
PVNCAP |
|
42.26 |
- |
| 10 |
Rudy, Lauren E (8)
|
PVNCAP |
41.28 |
42.50 |
1.22 |
| 11 |
Roth, Abigail B (8)
|
PVMACH |
43.77 |
43.09 |
-0.68 |
| 12 |
Cunningham, Emerson N (8)
|
PVTOLL |
|
43.90 |
- |
| 13 |
Brand, Emily N (8)
|
PVNCAP |
48.00 |
45.03 |
-2.97 |
| 14 |
Curry-Briggs, Sara E (8)
|
PVMACH |
46.39 |
46.93 |
0.54 |
| 15 |
Butler, Kaia M (8)
|
PVTOLL |
|
47.87 |
- |
| 16 |
Shin, Elizabeth J (8)
|
PVNCAP |
55.00 |
48.47 |
-6.53 |
| 17 |
Sarathy, Miraya (8)
|
PVMACH |
|
51.24 |
- |
| 18 |
Breeden, Elora C (8)
|
PVNCAP |
50.64 |
51.91 |
1.27 |
| 19 |
Ramey, Madeline E (8)
|
PVNCAP |
|
54.24 |
- |
| 20 |
Richadson, Paola M (8)
|
PVNCAP |
50.87 |
54.59 |
3.72 |
| 21 |
Schiffer, Molly C (8)
|
PVNCAP |
|
1:12.92 |
- |
|
Heiden, Madelyn K (8)
|
PVNCAP |
|
NS |
- |
|
Wilson, Jocelynn E (8)
|
PVNCAP |
|
NS |
- |