| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jackson, Mackenzie L (8)
|
PVMACH |
20.60 |
21.40 |
0.80 |
| 2 |
Qaasim, Khayirah N (8)
|
PVNCAP |
22.03 |
21.92 |
-0.11 |
| 3 |
Ramey, Madeline E (8)
|
PVNCAP |
23.85 |
22.53 |
-1.32 |
| 4 |
Cunningham, Emerson N (8)
|
PVTOLL |
25.47 |
23.33 |
-2.14 |
| 5 |
Zimmermann, Merick A (8)
|
PVMACH |
25.17 |
23.43 |
-1.74 |
| 6 |
Nichols, Eleanor M (8)
|
PVNCAP |
25.02 |
23.70 |
-1.32 |
| 7 |
Kim, Sophia D (8)
|
PVMAC |
23.89 |
24.89 |
1.00 |
| 8 |
Whisenant, Annabelle C (8)
|
PVNCAP |
22.84 |
25.08 |
2.24 |
| 9 |
Zellers, Julia S (8)
|
PVMACH |
|
25.29 |
- |
| 10 |
Rudy, Lauren E (8)
|
PVNCAP |
23.14 |
25.90 |
2.76 |
| 11 |
Grassmeyer, Charlotte W (8)
|
PVNCAP |
26.43 |
25.94 |
-0.49 |
| 12 |
Butler, Kaia M (8)
|
PVTOLL |
26.80 |
26.36 |
-0.44 |
| 13 |
Choi, Amber G (8)
|
PVMAC |
28.25 |
26.95 |
-1.30 |
| 14 |
Yoo, Delaney Z (8)
|
PVMACH |
27.37 |
28.38 |
1.01 |
| 15 |
McKoy, Gabriella M (8)
|
PVNCAP |
33.06 |
28.81 |
-4.25 |
| 16 |
Huffine-Wright, Olivia C (8)
|
PVJFD |
30.85 |
29.63 |
-1.22 |
| 17 |
Schiffer, Molly C (8)
|
PVNCAP |
33.68 |
33.91 |
0.23 |
| 18 |
Chewning, Claire W (8)
|
PVMACH |
|
54.49 |
- |
|
Wu, Michelle G (8)
|
PVMACH |
|
DQ |
- |
|
Wilson, Jocelynn E (8)
|
PVNCAP |
|
NS |
- |
|
Heiden, Madelyn K (8)
|
PVNCAP |
|
NS |
- |