| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nguyen, Blake R (12)
|
PVMACH |
49.04 |
44.27 |
-4.77 |
| 2 |
Burton, Bryce L (12)
|
PVMACH |
44.50 |
44.60 |
0.10 |
| 3 |
Bravery, Jackson H (11)
|
PVMACH |
44.89 |
44.92 |
0.03 |
| 4 |
Tranchemontagne, Daniel J (12)
|
PVMACH |
|
45.29 |
- |
| 5 |
Faustini, Nina M (12)
|
PVMACH |
48.05 |
45.66 |
-2.39 |
| 6 |
Harriot, Erik A (12)
|
PVMACH |
47.08 |
47.31 |
0.23 |
| 7 |
Flickinger, Ryan G (12)
|
PVMACH |
47.86 |
48.01 |
0.15 |
| 8 |
Warren, Charles T (12)
|
PVMACH |
1:00.10 |
48.45 |
-11.65 |
| 9 |
Rayson, Devin K (11)
|
PVMACH |
50.34 |
49.52 |
-0.82 |
| 10 |
Braccia, Eva K (12)
|
PVMACH |
|
54.40 |
- |
| 11 |
Taylor, Matthew T (11)
|
PVMACH |
54.99 |
56.91 |
1.92 |
|
Armstrong, Katelyn A (12)
|
PVMACH |
|
NS |
- |