| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cornett, Annalise E (18)
|
PVFISH |
54.39 |
55.18 |
0.79 |
| 2 |
Martin, Allison M (18)
|
PVFISH |
51.29 |
56.22 |
4.93 |
| 3 |
Sunderhauf, Skylar L (17)
|
PVFISH |
53.49 |
56.28 |
2.79 |
| 4 |
Wood, Augusta D (16)
|
PVUN |
55.70 |
57.22 |
1.52 |
| 5 |
Gregory, Ashley B (16)
|
PVFISH |
56.75 |
57.30 |
0.55 |
| 6 |
Larson, Emma E (17)
|
PVTANK |
56.01 |
57.91 |
1.90 |
| 7 |
Altenburg, Kathleen I (15)
|
PVMAKO |
57.91 |
58.23 |
0.32 |
| 8 |
Bailey, Caroline S (15)
|
PVYORK |
57.57 |
58.33 |
0.76 |
| 9 |
Creeks, Sofia A (15)
|
PVFISH |
56.76 |
58.59 |
1.83 |
| 10 |
Pinillos, Maria X (16)
|
PVTANK |
58.25 |
58.79 |
0.54 |
| 11 |
Rizzardi, Paulina A (15)
|
PVMAKO |
59.36 |
59.23 |
-0.13 |
| 12 |
Spence, Eleanor R (15)
|
PVMAKO |
1:01.66 |
59.68 |
-1.98 |
| 13 |
Williams, Madeleine P (18)
|
PVUN |
1:01.53 |
1:00.38 |
-1.15 |
| 14 |
Layton, Tori G (17)
|
PVMAKO |
1:00.67 |
1:00.77 |
0.10 |
| 15 |
Nanz, Kealani G (17)
|
PVTANK |
1:00.11 |
1:01.23 |
1.12 |
| 16 |
Guthrie, Sydney E (15)
|
PVFISH |
1:01.74 |
1:01.70 |
-0.04 |
| 17 |
Larow, Katherine G (17)
|
PVUN |
|
1:02.81 |
- |
| 18 |
Walden, Riley M (16)
|
PVTANK |
1:02.06 |
1:02.96 |
0.90 |
| 19 |
Wan, Alison (16)
|
PVFISH |
1:05.55 |
1:03.15 |
-2.40 |
| 20 |
Salomons, Catherine A (15)
|
PVSTJS |
1:02.51 |
1:03.50 |
0.99 |
| 21 |
McGowan, Genevieve E (15)
|
PVUN |
1:04.04 |
1:04.13 |
0.09 |
| 22 |
Jingan, Jinglin (15)
|
PVFISH |
1:03.43 |
1:04.77 |
1.34 |
| 23 |
Blasch, Marin T (17)
|
PVMAKO |
1:03.75 |
1:04.91 |
1.16 |
| 24 |
Saunders, Alicia B (15)
|
PVUN |
1:04.86 |
1:05.02 |
0.16 |
| 25 |
Xu, Caroline (15)
|
PVFISH |
1:07.60 |
1:06.50 |
-1.10 |
| 26 |
Kiernan, Madeleine J (15)
|
PVYORK |
1:10.02 |
1:07.39 |
-2.63 |
| 27 |
Staley, Ana M (15)
|
PVMAKO |
1:04.61 |
1:07.79 |
3.18 |
| 28 |
Morris, Brianna C (17)
|
PVUN |
|
1:09.73 |
- |
| 29 |
Hale, Laila P (15)
|
PVUN |
|
1:11.04 |
- |
|
Modder, Kathleen F (16)
|
PVFISH |
55.55 |
NS |
- |
|
McGinley, Regan M (15)
|
PVYORK |
1:01.66 |
NS |
- |