| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Kayleigh M (12)
|
PVSTJS |
31.65 |
31.96 |
0.31 |
| 2 |
Tibayan, Leryn S (11)
|
PVSTJS |
35.45 |
34.12 |
-1.33 |
| 3 |
Hooff, Loraine T (11)
|
PVSTJS |
34.06 |
34.55 |
0.49 |
| 4 |
Murphy, Caitlyn G (11)
|
PVMAKO |
39.13 |
38.16 |
-0.97 |
| 5 |
Lunn, Elise C (11)
|
PVSTJS |
41.07 |
38.29 |
-2.78 |
| 6 |
Colwill, Jacqueline E (11)
|
PVMAKO |
41.79 |
41.28 |
-0.51 |
| 7 |
Podoff, Alyssa S (11)
|
PVMAKO |
|
43.76 |
- |
| 8 |
Kislowski, Ashley M (11)
|
PVOCCS |
46.56 |
43.99 |
-2.57 |
| 9 |
Windmiller, Alexia J (12)
|
PVOCCS |
43.79 |
45.63 |
1.84 |
| 10 |
Gondek, Zoey G (11)
|
PVOCCS |
53.98 |
46.68 |
-7.30 |
| 11 |
Crowley, Charlotte M (11)
|
PVMAKO |
57.81 |
49.61 |
-8.20 |
| 12 |
Lugiai, McKenna G (11)
|
PVOCCS |
52.97 |
54.78 |
1.81 |
|
Byers, Emily K (11)
|
PVMAKO |
45.26 |
DQ |
- |
|
Sharafeldin, Leila B (12)
|
PVFISH |
|
NS |
- |
|
Warmka, Selah H (11)
|
PVSTJS |
|
NS |
- |