| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ferrari, Victoria A (8)
|
PVYORK |
43.33 |
38.16 |
-5.17 |
| 2 |
Springer, Summer A (8)
|
PVYORK |
50.76 |
39.74 |
-11.02 |
| 3 |
Nielsen, Maddie G (8)
|
PVYORK |
|
40.30 |
- |
| 4 |
Wang, Katherine A (7)
|
PVASA |
41.60 |
42.81 |
1.21 |
| 5 |
Reiner, Hadley K (8)
|
PVASA |
48.93 |
43.42 |
-5.51 |
| 6 |
Qin, Eva X (8)
|
PVASA |
47.58 |
43.82 |
-3.76 |
| 7 |
Thompson, Ivy P (8)
|
PVYORK |
|
44.02 |
- |
| 8 |
Bareis, Mary M (8)
|
PVYORK |
1:01.08 |
47.23 |
-13.85 |
| 9 |
Hansel, Lila M (8)
|
PVASA |
50.34 |
48.71 |
-1.63 |
| 10 |
Jordan, Brianna R (8)
|
PVYORK |
1:00.55 |
50.00 |
-10.55 |
| 11 |
O'Reilly, Teagan E (6)
|
PVYORK |
|
1:11.74 |
- |
| 12 |
Blanco, Sophia P (6)
|
PVYORK |
|
1:12.33 |
- |
|
Gharakhanian, Stella L (8)
|
PVYORK |
|
NS |
- |
|
Mamillapalli, Ruthvika S (6)
|
PVYORK |
|
NS |
- |