| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Watts, Emma C (13)
|
PVFISH |
2:30.99 |
2:33.47 |
2.48 |
| 2 |
Janczyk, Megan D (14)
|
PVMACH |
2:34.66 |
2:41.00 |
6.34 |
| 3 |
Bedford, Madison K (14)
|
PVNCAP |
2:50.11 |
2:45.22 |
-4.89 |
| 4 |
O'Hara, Victoria L (13)
|
PVNCAP |
2:54.89 |
2:46.68 |
-8.21 |
| 5 |
Dougherty, Jacqueline G (14)
|
PVFAA |
2:54.49 |
2:52.13 |
-2.36 |
| 6 |
Suma, Sydney M (13)
|
PVMACH |
2:58.96 |
2:54.08 |
-4.88 |
| 7 |
Morrison, Zoe A (13)
|
PVFAA |
3:06.94 |
2:55.31 |
-11.63 |
| 8 |
Leong, Natalie I (13)
|
PVFAA |
2:52.02 |
2:56.32 |
4.30 |
| 9 |
Nagle, Audrey R (13)
|
PVMACH |
3:00.92 |
2:56.43 |
-4.49 |
| 10 |
Morris, Payton E (14)
|
PVNCAP |
3:03.28 |
2:57.64 |
-5.64 |
| 11 |
Williams, Maeve A (14)
|
PVMACH |
2:46.86 |
2:58.92 |
12.06 |
| 12 |
Killian, Scarlett G (13)
|
PVFAA |
3:04.29 |
3:00.71 |
-3.58 |
| 13 |
Lim Yang, Nora D (13)
|
PVMACH |
|
3:03.21 |
- |
| 14 |
Park, Jenny (13)
|
PVMAC |
|
3:08.87 |
- |
| 14 |
Birkan, Selin S (14)
|
PVFISH |
3:28.93 |
3:08.87 |
-20.06 |
| 16 |
Ebaugh, Madison E (13)
|
PVMACH |
|
3:12.46 |
- |
| 17 |
Wierschke, Danielle J (13)
|
PVFAA |
3:24.08 |
3:17.01 |
-7.07 |
| 18 |
Kasemsuwannakaeth, Natcha (13)
|
PVMACH |
3:20.95 |
3:17.69 |
-3.26 |
| 19 |
Holmes, Sophie L (13)
|
PVNCAP |
|
3:47.70 |
- |
| 20 |
Lee, Ellen (14)
|
PVSSCT |
|
4:07.48 |
- |
|
Kim, Danielle A (14)
|
PVMAC |
|
NS |
- |
|
Seanor, Julianne M (14)
|
PVMACH |
2:47.16 |
NS |
- |
|
Shields, Helen K (13)
|
PVFISH |
|
NS |
- |