| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
In, Emily (12)
|
PVMAC |
31.60 |
32.19 |
0.59 |
| 2 |
Bass, Aniah R (13)
|
PVDCPR |
35.38 |
33.57 |
-1.81 |
| 3 |
Bui, An T (12)
|
PVMSSC |
35.13 |
33.58 |
-1.55 |
| 4 |
Pascuccio Gomez, Fiona (12)
|
PVDCPR |
37.53 |
34.29 |
-3.24 |
| 5 |
Udoye, Ariana O (12)
|
PVNCAP |
33.56 |
34.35 |
0.79 |
| 6 |
Alexander-Fettus, Helen H (13)
|
PVDCPR |
43.55 |
38.39 |
-5.16 |
| 7 |
Charles-Balthazar, Danariell (12)
|
PVNCAP |
38.52 |
40.09 |
1.57 |
| 8 |
Usquiano, Genesis D (12)
|
PVMSSC |
43.45 |
40.43 |
-3.02 |
| 9 |
Ferguson, Teagan E (13)
|
PVDCPR |
44.38 |
40.44 |
-3.94 |
| 10 |
Rippon, Tru M (13)
|
PVFAST |
46.70 |
40.73 |
-5.97 |
| 11 |
Dixon, Makayla N (12)
|
PVMSSC |
|
43.54 |
- |
| 12 |
Chang, Leah S (12)
|
PVFAST |
|
52.62 |
- |