| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Williams, Sena T (13)
|
PVFAST |
28.87 |
28.13 |
-0.74 |
| 2 |
Udoye, Ariana O (12)
|
PVNCAP |
28.50 |
28.82 |
0.32 |
| 3 |
Smith, Camille L (12)
|
PVFAST |
30.06 |
30.59 |
0.53 |
| 4 |
Rippon, Tru M (13)
|
PVFAST |
32.22 |
32.12 |
-0.10 |
| 5 |
Downs, Mia B (12)
|
PVFAST |
33.28 |
33.36 |
0.08 |
| 6 |
Diaz, Emma E (12)
|
PVFAST |
|
33.58 |
- |
| 7 |
Manigo-Truell dos Sa, Do (13)
|
PVDCPR |
37.36 |
34.53 |
-2.83 |
| 8 |
Usquiano, Genesis D (12)
|
PVMSSC |
35.26 |
34.73 |
-0.53 |
| 9 |
Guerrieri Brown, Giulia (12)
|
PVDCPR |
35.50 |
36.76 |
1.26 |
| 10 |
Annexstein-Cox, Jasmine S (12)
|
PVDCPR |
43.91 |
44.72 |
0.81 |
|
Amador-Williams, Elizabeth M (12)
|
PVHEAL |
36.13 |
NS |
- |