| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Feng, Shoshana (10)
|
PVDRAG |
46.50 |
43.17 |
-3.33 |
| 2 |
Ferullo, Blake (9)
|
PVRMSC |
47.56 |
44.94 |
-2.62 |
| 3 |
Xue, Katherine Y (10)
|
PVDRAG |
47.75 |
46.76 |
-0.99 |
| 4 |
Souberbielle, Louise M (10)
|
PVTOLL |
47.00 |
47.11 |
0.11 |
| 5 |
Leung, Alaina A (10)
|
PVASA |
49.39 |
50.04 |
0.65 |
| 6 |
Narayanan, Saniya (9)
|
PVASA |
54.70 |
50.09 |
-4.61 |
| 7 |
Zenebe, Maraki S (10)
|
PVASA |
49.83 |
50.48 |
0.65 |
| 8 |
Bice, Annabella Y (10)
|
PVPAC |
59.07 |
51.29 |
-7.78 |
| 9 |
Guo, Elena (9)
|
PVRMSC |
50.35 |
51.97 |
1.62 |
| 10 |
Reeves, Sachi M (9)
|
PVPAC |
|
54.35 |
- |
| 11 |
Schestopol, Celia C (9)
|
PVASA |
|
55.44 |
- |
| 12 |
Cristinzio, Anna C (9)
|
PVASA |
|
56.54 |
- |
| 13 |
Zimmerman, Mallory J (10)
|
PVTOLL |
56.39 |
56.91 |
0.52 |
| 14 |
Kim, Katie J (9)
|
PVRMSC |
58.32 |
57.71 |
-0.61 |
| 15 |
Jones, Syerah C (9)
|
PVPAC |
1:06.72 |
57.98 |
-8.74 |
| 16 |
Tian, Esther (9)
|
PVRMSC |
1:05.26 |
58.13 |
-7.13 |
| 17 |
Wu, Judy D (9)
|
PVRMSC |
1:05.31 |
58.89 |
-6.42 |
| 18 |
Hallissey, Shoshana R (9)
|
PVTOLL |
1:04.30 |
59.99 |
-4.31 |
| 19 |
Vallhonrat, Charlotte M (9)
|
PVASA |
|
1:01.95 |
- |
| 20 |
Kidwell, Caitlin N (9)
|
PVPAC |
|
1:17.48 |
- |
|
Cooper, Ava (9)
|
PVPAC |
1:01.28 |
DQ |
- |
|
Batty, Leah M (10)
|
PVPAC |
58.54 |
DQ |
- |