| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Killian, Renee H (10)
|
PVFAA |
32.50 |
32.63 |
0.13 |
| 2 |
Valdecanas, Savannah E (10)
|
PVDRAG |
33.39 |
32.97 |
-0.42 |
| 3 |
Cisse, Mouneissa (10)
|
PVRMSC |
37.79 |
37.96 |
0.17 |
| 4 |
Ferullo, Blake (9)
|
PVRMSC |
38.86 |
39.38 |
0.52 |
| 5 |
Hallissey, Shoshana R (9)
|
PVTOLL |
45.10 |
40.55 |
-4.55 |
| 6 |
Bice, Annabella Y (10)
|
PVPAC |
46.80 |
41.73 |
-5.07 |
| 7 |
Haddad, Julianne R (9)
|
PVASA |
44.56 |
43.97 |
-0.59 |
| 8 |
Lei, Sophie (9)
|
PVRMSC |
57.86 |
45.99 |
-11.87 |
| 9 |
Hanna, Suriel S (9)
|
PVASA |
51.83 |
47.50 |
-4.33 |
| 10 |
Kim, Katie J (9)
|
PVRMSC |
52.24 |
51.55 |
-0.69 |
| 11 |
Wu, Judy D (9)
|
PVRMSC |
|
51.77 |
- |
| 12 |
Cooper, Ava (9)
|
PVPAC |
|
56.20 |
- |
|
Hefferon, Louise A (9)
|
PVASA |
42.31 |
DQ |
- |
|
Cristinzio, Anna C (9)
|
PVASA |
|
NS |
- |