| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hammond, Hailey G (13)
|
PVNCAP |
24.94 |
25.11 |
0.17 |
| 2 |
Schwieters, Julie K (13)
|
PVRMSC |
25.31 |
25.34 |
0.03 |
| 3 |
Wells, Maecy J (13)
|
PVOCCS |
25.08 |
25.46 |
0.38 |
| 4 |
Gary, Morgan C (13)
|
PVNCAP |
26.23 |
25.67 |
-0.56 |
| 5 |
Grabowski, Arielle M (13)
|
PVOCCS |
26.45 |
25.85 |
-0.60 |
| 6 |
Nishnianidze, Anna (13)
|
PVRMSC |
25.77 |
25.92 |
0.15 |
| 7 |
Morse, Victoria D (13)
|
PVASA |
26.17 |
26.32 |
0.15 |
| 8 |
Levine, Lauren F (13)
|
PVNCAP |
26.97 |
26.42 |
-0.55 |
| 9 |
Augustine, Ashlyn G (13)
|
PVOCCS |
26.32 |
26.52 |
0.20 |
| 10 |
Caiazza-Blum, Alice Y (13)
|
PVRMSC |
27.33 |
26.53 |
-0.80 |
| 11 |
Robinette, Allison C (13)
|
PVOCCS |
26.38 |
27.00 |
0.62 |
| 12 |
Alston, Elizabeth D (13)
|
PVMACH |
26.76 |
27.45 |
0.69 |
| 13 |
Miranda, Gabrielle L (13)
|
PVMACH |
28.59 |
27.51 |
-1.08 |
| 14 |
Cotton, Rachel E (13)
|
PVNCAP |
26.68 |
27.74 |
1.06 |
| 15 |
Stahl, Rosaleagh A (13)
|
PVOCCS |
27.87 |
28.20 |
0.33 |
|
Schumacher, Karly R (13)
|
PVMACH |
27.55 |
NS |
- |
|
Picardo, Nina Karylle B (13)
|
PVOCCS |
26.43 |
NS |
- |
|
Dorsey, Charlotte M (13)
|
PVASA |
29.53 |
NS |
- |