| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Witt, Makenzie B (13)
|
PVNCAP |
26.27 |
25.63 |
-0.64 |
| 2 |
Depman, Megan A (13)
|
PVRMSC |
25.73 |
25.74 |
0.01 |
| 3 |
Heath, Briana L (13)
|
PVNCAP |
26.82 |
26.63 |
-0.19 |
| 4 |
Huang, Sophie L (13)
|
PVDRAG |
26.66 |
26.66 |
0.00 |
| 5 |
Li, Julia Z (13)
|
PVDRAG |
26.64 |
26.86 |
0.22 |
| 6 |
Holland, Addison C (13)
|
PVNCAP |
27.26 |
26.92 |
-0.34 |
| 7 |
McCarthy, Clara A (13)
|
PVAAC |
27.34 |
27.07 |
-0.27 |
| 8 |
Crown, Katherine D (13)
|
PVNCAP |
27.85 |
27.12 |
-0.73 |
| 9 |
Zalalutdinov, Darya H (13)
|
PVRMSC |
27.99 |
27.31 |
-0.68 |
| 10 |
Kampschror, Reagan N (13)
|
PVNCAP |
28.64 |
27.68 |
-0.96 |
| 11 |
Thompson, Claire M (13)
|
PVYORK |
28.05 |
28.00 |
-0.05 |
| 12 |
Cebrian, Francesca A (13)
|
PVDRAG |
27.35 |
28.03 |
0.68 |
|
Mathias, Olivia (13)
|
PVDRAG |
27.59 |
NS |
- |