| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cory, McKenzie L (10)
|
PVYORK |
44.14 |
39.91 |
-4.23 |
| 2 |
Busen, McKinley R (9)
|
PVYORK |
41.67 |
41.47 |
-0.20 |
| 3 |
Joung, Jessica J (9)
|
PVMAC |
42.26 |
42.64 |
0.38 |
| 4 |
Chong, Finley E (10)
|
PVTIBU |
46.63 |
43.40 |
-3.23 |
| 5 |
Schur, Gracelyn K (10)
|
PVASA |
45.35 |
43.64 |
-1.71 |
| 6 |
Gines, Victoria L (9)
|
PVPM |
46.55 |
44.90 |
-1.65 |
| 7 |
Watters, Caroline R (10)
|
PVFBST |
48.28 |
45.97 |
-2.31 |
| 8 |
Keating, Hannah M (10)
|
PVTIBU |
51.42 |
48.08 |
-3.34 |
| 9 |
Montilla, Jocelyn A (10)
|
PVPM |
47.52 |
48.70 |
1.18 |
| 10 |
Bhardwaj, Adya (9)
|
PVASA |
48.78 |
48.93 |
0.15 |
| 11 |
Penetrante, Leia M (10)
|
PVPM |
53.09 |
49.71 |
-3.38 |
| 12 |
Robinson, Alyssa C (9)
|
PVMSSC |
51.86 |
51.03 |
-0.83 |
| 13 |
Alvarado-Gallego, Mariana (10)
|
PVPM |
|
52.43 |
- |
|
Holder, LeeAnn M (9)
|
PVMSSC |
45.77 |
DQ |
- |
|
Dressendorfer, Carson E (10)
|
PVYORK |
45.80 |
DQ |
- |