| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spurlock, Sophie T (9)
|
PVDCPR |
40.95 |
40.48 |
-0.47 |
| 2 |
Li, MingZe (9)
|
PVNCAP |
50.03 |
42.23 |
-7.80 |
| 3 |
Reilly, Clara G (9)
|
PVNCAP |
48.99 |
43.05 |
-5.94 |
| 4 |
Allender, Cora J (10)
|
PVDCPR |
|
49.45 |
- |
| 5 |
Anthony, Yamari A (10)
|
PVERSC |
|
51.41 |
- |
| 6 |
Ryan, Noelia J (9)
|
PVDCPR |
|
51.50 |
- |
| 7 |
Kline, Isa M (10)
|
PVDCPR |
1:14.03 |
52.76 |
-21.27 |
| 8 |
Morse, Charlotte C (9)
|
PVDCPR |
|
53.15 |
- |
| 9 |
Claude, Briana A (10)
|
PVERSC |
1:09.03 |
53.38 |
-15.65 |
| 10 |
Beggiato, Aria L (9)
|
PVDCPR |
57.76 |
54.19 |
-3.57 |
| 11 |
Snyder, Lily H (10)
|
PVDCPR |
1:09.69 |
55.48 |
-14.21 |
| 12 |
Vogel, Talia M (10)
|
PVDCPR |
1:03.69 |
56.04 |
-7.65 |
| 13 |
Lipton, Leila S (9)
|
PVDCPR |
|
56.35 |
- |
| 14 |
Dunlop, Carissa A (9)
|
PVDCPR |
|
1:00.59 |
- |
| 15 |
Song, Aria G (9)
|
PVDCPR |
|
1:03.29 |
- |
| 16 |
Ifill, Skylar G (10)
|
PVERSC |
|
1:20.02 |
- |
|
Conley, Suri W (10)
|
PVDCPR |
|
DQ |
- |
|
Berges Voorhis, Kira K (10)
|
PVDCPR |
|
DQ |
- |