| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
White, Riley E (10)
|
SCYCSC |
|
42.25 |
- |
| 2 |
Burns, Caroline P (10)
|
SCYSSC |
46.82 |
42.36 |
-4.46 |
| 3 |
York, Lauren E (10)
|
SCTG |
47.61 |
46.60 |
-1.01 |
| 4 |
Van Ginhoven, Helen M (10)
|
SCTG |
50.42 |
47.13 |
-3.29 |
| 5 |
Walker, Zoey A (10)
|
SCYSSC |
50.05 |
48.08 |
-1.97 |
| 6 |
Burns, Elizabeth B (9)
|
SCYSSC |
49.84 |
48.24 |
-1.60 |
| 7 |
Friedl, Cecilia L (10)
|
SCTG |
|
49.09 |
- |
| 8 |
Christian, Abigail C (9)
|
SCYCSC |
1:09.44 |
50.55 |
-18.89 |
| 9 |
Patangan, Elanorah G (10)
|
SCYCSC |
|
53.19 |
- |
| 10 |
Lewis, Nina M (10)
|
SCTG |
50.08 |
53.68 |
3.60 |
| 11 |
Beason, Naomi A (8)
|
SCTG |
1:10.61 |
55.14 |
-15.47 |
| 12 |
Steinke, Sawyer M (10)
|
SCYCSC |
|
55.52 |
- |
| 13 |
Kilmon, Kenzie G (9)
|
SCNYST |
|
57.49 |
- |
| 14 |
Shellem, Addison P (8)
|
SCYCSC |
56.43 |
59.67 |
3.24 |
| 15 |
Stallone, Gianna F (10)
|
SCTG |
1:01.18 |
1:01.37 |
0.19 |
| 16 |
Ceneviva, Anna G (9)
|
SCYSSC |
|
1:08.07 |
- |
| 17 |
McWilliams, Abigail L (9)
|
SCYSSC |
|
1:08.08 |
- |
| 18 |
Rostin, McCrae S (6)
|
SCTG |
|
1:21.83 |
- |
| 19 |
Martin, Morgan N (8)
|
SCTG |
|
1:22.56 |
- |
|
Shellem, Aubrey P (7)
|
SCYCSC |
|
DQ |
- |
|
Reimer, Patterson R (6)
|
SCYSSC |
|
DQ |
- |
|
Wooten, Avery M (10)
|
SCYSSC |
|
DQ |
- |