| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clark jr, Shaun K (13)
|
VALAC |
25.48 |
25.49 |
0.01 |
| 2 |
Clark, Jaedyn K (13)
|
VALAC |
26.08 |
26.26 |
0.18 |
| 3 |
Rajapakse, Kyle (14)
|
VAGATR |
25.91 |
26.76 |
0.85 |
| 4 |
Barron, Asher N (14)
|
VALAC |
26.40 |
26.82 |
0.42 |
| 5 |
Stewart, Gunnar D (14)
|
VASMAC |
26.64 |
26.86 |
0.22 |
| 6 |
McMahon, III, Robert W (13)
|
VASMAC |
27.86 |
27.95 |
0.09 |
| 7 |
Straight, Jack D (14)
|
VASMAC |
28.06 |
28.24 |
0.18 |
| 8 |
Krasheninnikov, Albert (13)
|
VAGATR |
28.42 |
29.49 |
1.07 |
| 9 |
Hankins, Jackson D (13)
|
VASMAC |
32.53 |
29.68 |
-2.85 |
| 10 |
Larson, Dietrich A (14)
|
VASMAC |
|
30.72 |
- |
| 11 |
Murphy, Brayden D (13)
|
VASMAC |
|
34.31 |
- |