| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Oswald, Aiden C (12)
|
VASMAC |
28.65 |
27.20 |
-1.45 |
| 2 |
Yang, Chenghao (12)
|
VAGATR |
31.35 |
29.53 |
-1.82 |
| 3 |
Bowman, Henry E (11)
|
VASMAC |
30.73 |
30.32 |
-0.41 |
| 4 |
Maust, Samuel L (11)
|
VAGATR |
30.52 |
30.75 |
0.23 |
| 5 |
Xie, William (11)
|
VAGATR |
36.36 |
30.83 |
-5.53 |
| 6 |
Rockwell, Thomas D (11)
|
VASMAC |
31.25 |
31.05 |
-0.20 |
| 7 |
Straight, Christian B (12)
|
VASMAC |
32.19 |
32.01 |
-0.18 |
| 8 |
Coleman, Joseph J (12)
|
VAGATR |
40.64 |
32.26 |
-8.38 |
| 9 |
Dmytriyev, Boris (12)
|
VAGATR |
32.72 |
32.51 |
-0.21 |
| 10 |
Fish, Xavier L (12)
|
VASMAC |
33.59 |
32.68 |
-0.91 |
| 11 |
Kerrigan, Tyler W (11)
|
VAGATR |
34.63 |
32.70 |
-1.93 |
| 12 |
Kasten, Francis E (12)
|
VAGATR |
34.61 |
33.13 |
-1.48 |
| 13 |
Alhusen, Maximilian K (12)
|
VASMAC |
|
34.31 |
- |
| 14 |
Apicella, Ethan S (11)
|
VASMAC |
36.21 |
36.35 |
0.14 |
| 15 |
Thomas, Caleb P (12)
|
VAGATR |
44.48 |
43.30 |
-1.18 |
| 16 |
Patel, Shane D (11)
|
VASMAC |
|
44.64 |
- |
|
Milam, Jeb P (11)
|
VALAC |
32.08 |
NS |
- |