| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Francis, Lane M (11)
|
VANOVA |
30.63 |
30.00 |
-0.63 |
| 2 |
Bynum, Madeline S (11)
|
VANOVA |
31.66 |
30.33 |
-1.33 |
| 3 |
Liu, Ashley C (11)
|
PVNCAP |
31.58 |
30.77 |
-0.81 |
| 4 |
Dmochowski, Vivian Louise S (11)
|
PVNCAP |
33.45 |
32.38 |
-1.07 |
| 5 |
Davy-Brown, Layla S (11)
|
PVNCAP |
32.77 |
32.55 |
-0.22 |
| 6 |
Tang, Natalie J (11)
|
VANOVA |
32.80 |
32.99 |
0.19 |
| 7 |
Edwards, Amelia L (11)
|
PVNCAP |
34.06 |
33.13 |
-0.93 |
| 8 |
Lande, Charlotte A (11)
|
VANOVA |
35.21 |
34.78 |
-0.43 |
| 9 |
Tom, Kaitlyn L (11)
|
MRLIAC |
34.99 |
34.81 |
-0.18 |
| 10 |
Siegel, Alexandra R (11)
|
MRLIAC |
36.01 |
35.18 |
-0.83 |
| 11 |
Canales Alvizuri, Isabella N (11)
|
MRLIAC |
35.72 |
35.46 |
-0.26 |
| 12 |
Stertsios, Martha M (11)
|
MRLIAC |
39.72 |
38.90 |
-0.82 |