| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeGuenther, Emory B (13)
|
VANOVA |
57.55 |
53.30 |
-4.25 |
| 2 |
Anderson, Samantha R (13)
|
MRLIAC |
54.86 |
53.96 |
-0.90 |
| 3 |
Hammond, Hailey G (13)
|
PVNCAP |
53.15 |
54.11 |
0.96 |
| 4 |
Clattenburg, Hannah C (13)
|
PVNCAP |
57.26 |
55.25 |
-2.01 |
| 5 |
Wilbur, Lilla E (13)
|
PVNCAP |
55.46 |
55.47 |
0.01 |
| 6 |
Koroma, Sophie I (13)
|
PVNCAP |
56.58 |
55.87 |
-0.71 |
| 7 |
Derrico, Campbell K (13)
|
VANOVA |
57.77 |
56.79 |
-0.98 |
| 8 |
Carvalho, Gabriela (13)
|
VANOVA |
55.74 |
57.01 |
1.27 |
| 9 |
Laramore, Ellie B (13)
|
VANOVA |
58.00 |
57.69 |
-0.31 |
| 10 |
Leung, Megan S (13)
|
MRLIAC |
1:01.28 |
1:01.13 |
-0.15 |