| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bynum, Madeline S (11)
|
VANOVA |
27.49 |
26.23 |
-1.26 |
| 2 |
Harris, Savannah J (11)
|
VANOVA |
28.46 |
27.34 |
-1.12 |
| 3 |
Deigan, Charlotte J (11)
|
PVNCAP |
27.45 |
27.68 |
0.23 |
| 4 |
Gavino, Alessandra B (11)
|
PVNCAP |
29.06 |
28.68 |
-0.38 |
| 5 |
Davy-Brown, Layla S (11)
|
PVNCAP |
28.13 |
28.82 |
0.69 |
| 6 |
Liu, Ashley C (11)
|
PVNCAP |
28.94 |
28.88 |
-0.06 |
| 7 |
Hull, Elliana G (11)
|
VANOVA |
30.05 |
29.70 |
-0.35 |
| 8 |
Canales Alvizuri, Isabella N (11)
|
MRLIAC |
30.13 |
30.28 |
0.15 |
| 9 |
Bonham, Abigail A (11)
|
VANOVA |
32.88 |
30.73 |
-2.15 |
| 10 |
Polanco, Maryann (11)
|
MRLIAC |
32.70 |
31.28 |
-1.42 |
| 11 |
Kuo, Leila Y (11)
|
MRLIAC |
30.69 |
32.32 |
1.63 |
| 12 |
Stertsios, Martha M (11)
|
MRLIAC |
33.71 |
33.27 |
-0.44 |