| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jacobs, Lane E (10)
|
VANOVA |
33.31 |
32.66 |
-0.65 |
| 2 |
Phadtare, Ashlesha A (10)
|
VANOVA |
34.33 |
33.86 |
-0.47 |
| 3 |
Lee, Christina S (10)
|
PVNCAP |
36.09 |
34.93 |
-1.16 |
| 4 |
Anderson, Kasey R (10)
|
MRLIAC |
34.65 |
35.27 |
0.62 |
| 5 |
Kutner, Virginia T (10)
|
MRLIAC |
36.34 |
35.48 |
-0.86 |
| 6 |
Hepper, Margaret C (10)
|
VANOVA |
36.20 |
35.54 |
-0.66 |
| 7 |
Shifflett, Jillian M (10)
|
PVNCAP |
36.17 |
35.95 |
-0.22 |
| 8 |
Schroeder, Elizabeth C (10)
|
PVNCAP |
36.63 |
36.19 |
-0.44 |
| 9 |
Kohner, Charlotte A (10)
|
MRLIAC |
38.60 |
36.87 |
-1.73 |
| 10 |
Cushman, Addison K (10)
|
PVNCAP |
37.16 |
37.00 |
-0.16 |
| 11 |
Goins, Rebecca C (10)
|
VANOVA |
37.81 |
37.01 |
-0.80 |
| 12 |
Wu, Georgia M (10)
|
MRLIAC |
39.04 |
39.07 |
0.03 |