| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Danahy, John E (10)
|
VANOVA |
31.79 |
31.95 |
0.16 |
| 2 |
Linkonis, Richard P (10)
|
VANOVA |
32.95 |
32.11 |
-0.84 |
| 3 |
Meehan, Michael W (10)
|
MRLIAC |
34.72 |
34.22 |
-0.50 |
| 4 |
Schoch, William E (10)
|
PVNCAP |
35.24 |
34.47 |
-0.77 |
| 5 |
Song, Aaron J (10)
|
PVNCAP |
35.33 |
34.48 |
-0.85 |
| 6 |
Mollerup, Landon J (10)
|
PVNCAP |
34.81 |
35.68 |
0.87 |
| 7 |
Yun, Jayden C (10)
|
MRLIAC |
36.42 |
35.88 |
-0.54 |
| 8 |
Wong, Preston S (10)
|
VANOVA |
35.14 |
35.97 |
0.83 |
| 9 |
Mohammad, Alieldien S (10)
|
MRLIAC |
35.99 |
35.98 |
-0.01 |
| 10 |
Phillips, Logan J (10)
|
PVNCAP |
36.14 |
36.29 |
0.15 |
| 11 |
Connors, Christopher J (10)
|
VANOVA |
36.84 |
36.57 |
-0.27 |
| 12 |
Quach, Andrew (10)
|
MRLIAC |
36.98 |
36.94 |
-0.04 |