| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moore, Caitlyn N (10)
|
VANOVA |
32.13 |
31.13 |
-1.00 |
| 2 |
Phadtare, Ashlesha A (10)
|
VANOVA |
33.22 |
33.44 |
0.22 |
| 3 |
Jacobs, Lane E (10)
|
VANOVA |
34.54 |
34.08 |
-0.46 |
| 4 |
Laraway, Ellie M (9)
|
VANOVA |
36.30 |
34.96 |
-1.34 |
| 5 |
Duke, Avery T (10)
|
VANOVA |
35.47 |
35.10 |
-0.37 |
| 6 |
Hepper, Margaret C (10)
|
VANOVA |
37.68 |
35.48 |
-2.20 |
| 7 |
Goins, Rebecca C (10)
|
VANOVA |
38.54 |
37.23 |
-1.31 |
| 8 |
Blackston, Anna M (9)
|
VANOVA |
42.40 |
37.92 |
-4.48 |
| 9 |
Darden, Audrey G (10)
|
VANOVA |
40.59 |
39.85 |
-0.74 |
| 10 |
Vogel, Hannah L (9)
|
VANOVA |
44.60 |
41.81 |
-2.79 |
| 11 |
Porter, Anna J (10)
|
VANOVA |
1:07.20 |
42.10 |
-25.10 |
| 12 |
Paget, Julia A (10)
|
VANOVA |
44.24 |
42.45 |
-1.79 |
| 13 |
Ehrensberger, Margaret C (9)
|
VANOVA |
49.74 |
48.01 |
-1.73 |
| 14 |
Francis, Nora M (10)
|
VANOVA |
47.75 |
48.32 |
0.57 |