| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Addleman, Paige N (12)
|
MDUN |
30.89 |
29.58 |
-1.31 |
| 2 |
Pruski, Clara C (12)
|
MDUN |
30.59 |
30.93 |
0.34 |
| 3 |
Meyer, Amelie G (11)
|
VAACAC |
30.79 |
30.97 |
0.18 |
| 4 |
Spalding, Elizabeth E (12)
|
VASRVA |
34.16 |
34.09 |
-0.07 |
| 5 |
Harton, Margaret R (11)
|
VASRVA |
34.03 |
34.57 |
0.54 |
| 6 |
Stupar, Aliyah G (12)
|
VACGBD |
35.41 |
35.40 |
-0.01 |
| 7 |
Landon, Emma M (12)
|
VACVAC |
35.67 |
35.67 |
0.00 |
| 8 |
Baker, Haley K (11)
|
VASRVA |
34.42 |
36.16 |
1.74 |
| 9 |
Archer, Zoe R (12)
|
VACGBD |
39.05 |
37.85 |
-1.20 |
| 10 |
DeGaramo, Peyton E (11)
|
VACVAC |
38.81 |
38.53 |
-0.28 |
| 11 |
Burton, Leah L (11)
|
VACGBD |
|
39.14 |
- |
| 12 |
Seaborn, Mary A (12)
|
VASRVA |
44.31 |
39.55 |
-4.76 |
| 13 |
Rose, Sydney B (11)
|
VASRVA |
|
39.84 |
- |
| 14 |
Walker, Madison N (11)
|
VASRVA |
39.75 |
39.86 |
0.11 |
| 15 |
Karasiewicz, Austyn S (11)
|
MDBAY |
41.32 |
39.93 |
-1.39 |
| 16 |
Brochu, Emily C (12)
|
VACVAC |
46.62 |
41.43 |
-5.19 |
| 17 |
Simeral, Delaney K (11)
|
VACGBD |
55.86 |
41.73 |
-14.13 |
| 18 |
Willing, Julia D (11)
|
VASRVA |
|
42.05 |
- |
| 19 |
Felder, Alyssa K (12)
|
MDBAY |
45.36 |
43.22 |
-2.14 |
| 20 |
Gilley, Caroline J (11)
|
VASRVA |
|
48.21 |
- |
|
Cuce, Elianna G (11)
|
VASRVA |
39.57 |
NS |
- |