| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nolde, Anna E (8)
|
VAACAC |
45.61 |
44.32 |
-1.29 |
| 2 |
Karasiewicz, Hayden A (7)
|
MDBAY |
50.52 |
50.26 |
-0.26 |
| 3 |
Raeburn, Morgan Delaney (7)
|
VACVAC |
51.53 |
51.80 |
0.27 |
| 4 |
Schwarzenberg-Word, Alia (8)
|
VACGBD |
59.66 |
52.44 |
-7.22 |
| 5 |
Pitts, Caitlin W (8)
|
MDBAY |
|
55.42 |
- |
| 6 |
Meledick, Hannah R (7)
|
MDBAY |
1:03.65 |
58.45 |
-5.20 |
| 7 |
Shipe, Chloe E (8)
|
VACGBD |
1:14.75 |
59.28 |
-15.47 |
| 8 |
Greer, Ashante L (7)
|
VASRVA |
1:02.10 |
1:00.61 |
-1.49 |
| 9 |
Lewis, Reagan Q (8)
|
MDBAY |
1:06.82 |
1:01.83 |
-4.99 |
| 10 |
Quick, Leila K (8)
|
VACVAC |
1:08.21 |
1:05.67 |
-2.54 |
|
Rodgers, Sadie M (8)
|
VASRVA |
58.67 |
NS |
- |