| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lawson, Kristopher R (15)
|
PVNCAP |
50.57 |
51.16 |
0.59 |
| 2 |
Kelly, Jordan M (15)
|
PVNCAP |
51.74 |
52.48 |
0.74 |
| 3 |
Matsuyama, Neo J (16)
|
PVNCAP |
54.22 |
54.23 |
0.01 |
| 4 |
Hammoud, Ali B (18)
|
PVNCAP |
53.84 |
54.44 |
0.60 |
| 5 |
Vittori, Enrico (16)
|
PVNCAP |
53.61 |
54.63 |
1.02 |
| 6 |
Pham, Peter E (18)
|
VAVSTP |
54.15 |
55.46 |
1.31 |
| 7 |
Kim, Junwoo C (15)
|
PVNCAP |
56.19 |
56.18 |
-0.01 |
| 8 |
Friday, Jalen C (17)
|
PVNCAP |
55.75 |
56.43 |
0.68 |
| 9 |
Wu, Matthew J (15)
|
PVNCAP |
1:00.47 |
57.59 |
-2.88 |
| 10 |
Mallios, Braden G (16)
|
PVNCAP |
57.81 |
58.10 |
0.29 |
| 11 |
Sonne, William C (17)
|
PVNCAP |
54.84 |
58.51 |
3.67 |
| 12 |
Cram, Trevor C (18)
|
VAVSTP |
57.26 |
58.61 |
1.35 |
| 13 |
Boucree, Christopher J (15)
|
PVNCAP |
59.66 |
58.78 |
-0.88 |
| 14 |
Harrison, Joshua D (15)
|
PVNCAP |
1:04.52 |
1:01.05 |
-3.47 |
| 15 |
Post, Aidan J (17)
|
VAVSTP |
58.79 |
1:01.33 |
2.54 |
| 16 |
Connor, Daniel K (15)
|
VAVSTP |
1:04.73 |
1:03.07 |
-1.66 |
|
Beaudoin, Benjamin R (17)
|
VAVSTP |
53.22 |
NS |
- |
|
Nurkic Kacapor, Mak E (15)
|
PVNCAP |
59.76 |
NS |
- |