| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hammond, Hailey G (13)
|
PVNCAP |
57.30 |
54.43 |
-2.87 |
| 2 |
Orlic, Anastasia L (13)
|
PVNCAP |
1:00.47 |
59.95 |
-0.52 |
| 3 |
Knight, Anna S (14)
|
PVNCAP |
59.79 |
1:00.09 |
0.30 |
| 4 |
Alisbah, Lindsay K (14)
|
PVNCAP |
1:00.26 |
1:00.13 |
-0.13 |
| 5 |
Reed, Madison N (14)
|
VAVSTP |
57.09 |
1:00.15 |
3.06 |
| 6 |
Weliwitigoda, Sonia P (13)
|
PVNCAP |
1:01.37 |
1:00.69 |
-0.68 |
| 7 |
Holley, Palmer S (14)
|
PVNCAP |
55.56 |
1:01.25 |
5.69 |
| 8 |
Defibaugh, Gracie L (14)
|
VAVSTP |
59.60 |
1:01.26 |
1.66 |
| 9 |
Naum, Katherine S (14)
|
PVNCAP |
58.67 |
1:01.64 |
2.97 |
| 10 |
Hua, Annie T (14)
|
VAVSTP |
1:04.01 |
1:04.56 |
0.55 |
| 11 |
Beaudoin, Isabella A (13)
|
VAVSTP |
1:06.46 |
1:09.02 |
2.56 |