| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hammond, Hailey G (13)
|
PVNCAP |
25.82 |
24.94 |
-0.88 |
| 2 |
Knight, Anna S (14)
|
PVNCAP |
27.07 |
26.75 |
-0.32 |
| 3 |
Holley, Palmer S (14)
|
PVNCAP |
25.72 |
26.85 |
1.13 |
| 4 |
Reed, Madison N (14)
|
VAVSTP |
26.43 |
27.56 |
1.13 |
| 5 |
Naum, Katherine S (14)
|
PVNCAP |
27.26 |
28.18 |
0.92 |
| 6 |
Orlic, Anastasia L (13)
|
PVNCAP |
29.49 |
28.21 |
-1.28 |
| 7 |
Gaskin, Catherine A (14)
|
PVNCAP |
27.87 |
28.31 |
0.44 |
| 8 |
Defibaugh, Gracie L (14)
|
VAVSTP |
27.80 |
28.42 |
0.62 |
| 9 |
Weliwitigoda, Sonia P (13)
|
PVNCAP |
28.23 |
28.43 |
0.20 |
| 10 |
Alisbah, Lindsay K (14)
|
PVNCAP |
27.89 |
28.60 |
0.71 |
| 11 |
Beaudoin, Isabella A (13)
|
VAVSTP |
31.20 |
30.10 |
-1.10 |
| 12 |
LaDuca, Deanna M (13)
|
VAVSTP |
30.36 |
30.42 |
0.06 |