| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ohtani, Haruki (10)
|
MDCAA |
2:49.51 |
2:41.90 |
-7.61 |
| 2 |
Strickland, Owen M (10)
|
PVNCAP |
2:39.41 |
2:44.05 |
4.64 |
| 3 |
Dolezel, Erik W (10)
|
MDCAA |
2:51.21 |
2:46.65 |
-4.56 |
| 4 |
Fernandez, Lukas J (10)
|
MDCAA |
3:01.94 |
2:55.57 |
-6.37 |
| 5 |
Jacobs, Lucas J (10)
|
MDMAC |
3:02.43 |
3:04.44 |
2.01 |
| 6 |
Suprun, Maximilian E (10)
|
MDCAA |
3:13.34 |
3:05.90 |
-7.44 |
| 7 |
De Los Reyes-Kundey, Aden A (10)
|
MDMAC |
3:07.57 |
3:07.12 |
-0.45 |
| 8 |
Hughes, Conner G (9)
|
VAWST |
3:15.04 |
3:11.37 |
-3.67 |
| 9 |
DeMarco, Alexander M (9)
|
MDMAC |
3:09.03 |
3:12.66 |
3.63 |
| 10 |
Voas, Elliot D (10)
|
MDMAC |
3:16.87 |
3:13.56 |
-3.31 |
| 11 |
DeVito, Jonathan T (9)
|
PVNCAP |
3:38.15 |
3:15.74 |
-22.41 |
| 12 |
Zhong, Bryan (9)
|
MDMAC |
3:12.47 |
3:19.05 |
6.58 |
| 13 |
Damian, Vlad (10)
|
PVHACC |
|
3:24.08 |
- |
| 14 |
Hill, Wesley J (9)
|
VAHNVR |
3:24.96 |
3:27.39 |
2.43 |
| 15 |
Croxton, Alden R (10)
|
VAHNVR |
3:14.66 |
3:29.40 |
14.74 |
| 16 |
Otten, Kellan P (10)
|
MDCAA |
3:55.47 |
3:31.79 |
-23.68 |
| 17 |
Swafford, Edward P (9)
|
MDMAC |
3:56.32 |
3:31.94 |
-24.38 |
| 18 |
Franz, Brice R (9)
|
VAWST |
3:59.12 |
3:33.81 |
-25.31 |
| 19 |
Ballentine, Troy A (9)
|
MDMAC |
3:40.00 |
3:38.70 |
-1.30 |
| 20 |
Yaselli, Kevin J (10)
|
MDMAC |
4:12.55 |
3:45.58 |
-26.97 |
| 21 |
Sutherland, Reid A (9)
|
MDMAC |
4:20.64 |
3:58.24 |
-22.40 |
| 22 |
Zumbrunn, Zachary D (10)
|
MDMAC |
3:50.99 |
4:16.41 |
25.42 |