| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Woodruff, Austin C (8)
|
VALY |
45.17 |
44.04 |
-1.13 |
| 2 |
Arcarese, William M (8)
|
VANOVA |
47.81 |
44.16 |
-3.65 |
| 3 |
Tan, Zander (8)
|
VANOVA |
45.63 |
45.24 |
-0.39 |
| 4 |
Abbott, Trent M (8)
|
VANOVA |
43.95 |
45.48 |
1.53 |
| 5 |
Kiser, Harrison J (8)
|
VAGATR |
44.82 |
47.10 |
2.28 |
| 6 |
Hill, Riggins C (8)
|
VAGATR |
39.91 |
47.52 |
7.61 |
| 7 |
Du, Abraham B (8)
|
VAHOKI |
48.58 |
49.19 |
0.61 |
| 8 |
Payne, Matthew B (7)
|
VANOVA |
51.76 |
53.91 |
2.15 |
| 9 |
Peat, Cole H (7)
|
VANOVA |
54.89 |
54.82 |
-0.07 |
| 10 |
Worthington, Breckin K (8)
|
VANOVA |
58.28 |
56.08 |
-2.20 |
| 11 |
Cho, Nathan K (6)
|
VANOVA |
55.51 |
58.15 |
2.64 |
| 12 |
Banks, Logan M (8)
|
VANOVA |
1:01.23 |
1:00.65 |
-0.58 |
| 13 |
Kite, Elias G (7)
|
VAGATR |
1:08.57 |
1:05.39 |
-3.18 |
| 14 |
Barcia, Prince X (8)
|
VACA-Y |
1:02.09 |
1:06.63 |
4.54 |
| 15 |
Waterworth, William T (8)
|
VANOVA |
1:07.54 |
1:12.36 |
4.82 |